Figure 4.

MiR-7 mitigates the MPP⁺-induced neuronal apoptosis by targeting Sirt2. The SH-SY5Y cells with or without MPP⁺ treatment were transfected with RNA oligonucleotides or with or without plasmid pcDNA3.1-Sirt2. (A) Western blot analyses of Sirt2 and Bim expression. β-actin was used as a control. (B, C) Relative protein band densities of Sirt2 (B) and Bim (C). (D) Caspase 3 activity assay for detection of neuronal apoptosis. Representative Western blots are shown. Data are represented as the mean ± SD of three replicates. *P< 0.05.