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. 2016 Feb 15;8(2):993–1004.

Figure 5.

Figure 5

Restoration of Bax counteracts the inhibitory effects of miR-7 on MPP+-induced neuronal apoptosis. The SH-SY5Y cells with or without MPP+ treatment were transfected with RNA oligonucleotides or with or without plasmid pcDNA3.1-Bax. (A-C) The nucleosomal fragmentation (A), MMP (B), and caspase 3 activity (C) assays of the cells transfected with miR-7 or miR-NC showing the effects on apoptosis of mock-treated or MPP+-treated SH-SY5Y cells with or without introduction of Bax. (D) Western blot analyses of Bcl-2, Bax, Cl-caspase 3, and Cyt c expression. β-actin was used as control. Representative Western blots are shown. Data are represented as the mean ± SD of three replicates. *P< 0.05, and **P< 0.01.