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Silencing of VEGF expression promote apoptosis of SaOS-2 cells. A. Expression of Bax mRNA was assayed by RT-PCR in SaOS-2 cells, **p < 0.01. B. The cell apoptotic rate was cauculated by flow-cytometry (FCM) in SaOS-2 cells after treated with Ad-VEGF-siRNA-1 and Ad-VEGF-siRNA-3 for 24 h, PBS and/or Ad-Control-siRNA group cells were considered as normalizion, ***p < 0.001. C. The representative images were shown in FCM detection.
