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. 2016 Feb 15;8(2):1144–1154.

Figure 4.

Figure 4

Changes in spinal MrgC, Gi, NR2B, p-NR2B, t-nNOS and p-nNOS protein expression at different time points after intrathecal injection of BAM8-22 and anti-MrgC on day 14 after operation (n = 6 each). (A) Protein expression of MrgC, Gi, t-NR2B, p-NR2B, t-nNOS and p-nNOS assessed using Western blotting. β-actin was used as a loading control. Quantification of the normalized OD of (B) MrgC/β-actin, (C) Gi/β-actin, (D) t-NR2B/β-actin, (E) nNOS/β-actin, (F) p-NR2B/β-actin, and (G) p-nNOS/β-actin. *, P < 0.05 compared with sham + vehicle group; #, P < 0.05 compared with BCP + vehicle group.