Figure 4.

The anti-cancer effects of STO-609 are not AMPK-dependent. (A-C) Western blots demonstrating the effects of STO-609 on phosphorylation of Akt and ERK (A), apoptosis (B) and autophagy (C) in control or ‘AMPK-silenced’ gastric cancer cells. (D and E) apoptosis/necrosis examination of control or AMPK- and CaMKK-β-‘silenced’ gastric cancer cells incubated with 10 μM STO-609. DMSO, a vehicle of STO-609, was employed as a control. *P ≤ 0.001. Results are representative of three individual experiments.