Table 1.
Primers | Denaturation | 94°C for 5 min | |
---|---|---|---|
LPR | F: 5’-GGC GTT GCC GCT CTG AAT GC-3’ | 35 cycles | 94°C for 30 sec |
R: 5’-GAG GGA CTG AGC TGG ACA ACC AC-3’ | 60°C for 30 sec | ||
72°C for 60 sec | |||
VNTR | F: 5’-GTC AGT ATC ACA GGC TGC GAG-3’ | 30 cycles | 94°C for 30 sec |
R: 5’-TGT TCC TAG TCT TAC GCC AGT G-3’ | 58°C for 40 sec | ||
72°C for 45 sec | |||
Extension | 72°C for 10 min |