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. 2016 Feb 15;8(2):1281–1292.

Table 1.

Conditions of PCR

Primers Denaturation 94°C for 5 min
LPR F: 5’-GGC GTT GCC GCT CTG AAT GC-3’ 35 cycles 94°C for 30 sec
R: 5’-GAG GGA CTG AGC TGG ACA ACC AC-3’ 60°C for 30 sec
72°C for 60 sec
VNTR F: 5’-GTC AGT ATC ACA GGC TGC GAG-3’ 30 cycles 94°C for 30 sec
R: 5’-TGT TCC TAG TCT TAC GCC AGT G-3’ 58°C for 40 sec
72°C for 45 sec
Extension 72°C for 10 min