Fig. 2.

Analysis to determine a T. cruzi infected cell on the Operetta imaging system using building blocks in Harmony software. (A) Overlay of Hoechst and HCS CellMask Green fluorescent stains (B) Detection of the nucleus of host cells (C) Spot detection to define a parasite (one amastigote = 1 spot). (D) Expanded section of (C) to show the effectiveness of spot detection in identifying parasites within the host cell. P = parasite nucleus; H = Host cell nucleus. Scale bars represent 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)