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. 2015 Oct 14;5(3):215–228. doi: 10.1016/j.ijpddr.2015.10.001

Fig. 5.

Fig. 5

The linearity of the relationship between the MOI and the resulting number of T. cruzi infected 3T3 fibroblasts per well following 48 h post infection, determined on the Operetta and Opera image-based systems. (A) at 20–40:1 MOI, there appears to be a maximum number of host cells infected (B) a linear relationship was found up to a multiplicity of 5:1 parasite to host cell. Representative data is from one experiment, with 28 wells per MOI. R2 = 0.99.