Fig. 7.

Identification of T. cruzi infected 3T3 cells using the Opera QEHS high-content imaging system. Images were taken at 20× water magnification. (A) infected host cells. Green represents the host cell cytoplasm detected with HCS CellMask Green. Blue depicts nuclear material from both host cells and parasite stained with Hoechst. (B) Definition of the cytoplasmic region of host cells in which spot detection of parasite nuclei is undertaken. (C) Spot detection to define parasite nuclei. (D) Application of script using the Opera assay language interface to define infected cells. Infected cells are green, red are non-infected. (E–H) follows the same definitions for (A–D), only that these are infected host cells that have been treated with 12 μM NFX for 48 h. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)