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. 2016 Apr 27;6:24896. doi: 10.1038/srep24896

Figure 3. RTqPCR versus sequencing quantification of miRNA expression.

Figure 3

(A) Ratios of miR-7a-5p, miR-24-3p, miR-29-3p, miR-137-3p and miR-1843-5p expression to miR-124-3p expression at P4, P8, P14.4 and P21.4 were compared to ratios at P28 using data recovered from HTS or RT-qPCR technologies. Data are organized in lines (one miRNA/line) and columns (one stage/column). The reference ratio of 1 at P28 is shown as black bars while ratios at P4, P8, P14.4, P21.3 and P21.4 are shown as grey bars. p-values < 0.05, 0.01 and 0.001 are identified by *,** and *** symbols, respectively. All data are expressed as mean +/− SEM. (B) Ratios of miR-7a-5p, miR-24-3p, miR-29-3p, miR-137-3p and miR-1843-5p expression to miR-124-3p expression were calculated at P28 for progeny born to dams fed a balanced diet and compared to those of progeny born to dams fed an unbalanced diet.