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. 2016 Apr 27;6:24913. doi: 10.1038/srep24913

Figure 6. Involvement of the reticuloendothelial system in protection against Stx2 by (2vb27)2-SA.

Figure 6

(A) Depletion of splenic macrophages by Lip-Clod treatment. Adult BALB/c mice (3–4 mice/group) were injected i.v. with a unique dose of 200 μl of Lip-Clod or Lip-PBS. Twenty-four hours later, the animals were injected i.p. with 25 μl (diluted 1:4 in PBS) of a rabbit anti-mouse platelet antiserum. Five hours after antibody inoculation, mice were bled and platelets were counted in a veterinary automatic hematology analyzer. ***p < 0.001. NS: not significant. ANOVA. (B) Protection of (2vb27)2-SA against rStx2 in the absence of splenic macrophages. Adult BALB/c mice (3 mice/group) were i.v. injected with of 200 μl of Lip-Clod or Lip-PBS. Twenty-four hours later, mice were i.v. injected with 1LD100 of rStx2 pre-incubated 1 h at 37 °C with or without 1 pmol/mouse of (2vb27)2-SA. *p < 0.05 vs Lip-PBS Stx2 and Lip-Clod Stx2. Log-Rank Test.