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. 2016 Apr 27;6:24970. doi: 10.1038/srep24970

Figure 7. PrPΔ214–229 is retained in the Golgi after Thapsigargin treatment and p38-MAPK phosphorylation is increased.

Figure 7

(a) N2a cells were treated with Thapsigargin (TG) for 10 min and fixed at different time points. PrPΔ214–229 (green) is found mainly in the ER (colocalizing with PDI; red) at steady-state but after 30 min it is found in the cis-Golgi (colocalizing with GM130; red) traveling to the trans-Golgi network (colocalizing with TGN46; red) after 60 min. At 120 min PrPΔ214–229 is found mainly colocalizing with GM130 again. At this time point, some degree of colocalization (inset) of PrPΔ214–229 with the lyosomal marker LAMP1 (red) could be observed. In steady-state conditions WTPrPC is mainly found in the Golgi (colocalizing with GM130; red) and at the plasma membrane. At 60 and 120 min after TG treatment the intensity of the staining is decreased and WTPrPC is found at the plasma membrane but not in Golgi anymore. Scale bar is 10 μm. (b) Representative Western blot showing that, at steady-state, there is a high level of phosphorylation of p38-MAPK in PrPΔ214–229 compared to WTPrPC that decreases between 15–60 min to increase again at 120 min (*p < 0.05).