Chromatograms obtained of purified extracts from chicory cv. Spadona (A) and cv. Puna II (B) used for in vitro assays with free-living and parasitic stages of Ostertagia ostertagi. Chromatograms are presented at the wavelength where isosbestic points were detected for each extract (271 nm for Spadona and 275 nm for Puna II). 3 = 11, 13-dihydro-lactucin; 4 = lactucin; 6 = 8-deoxy-lactucin; 7 = 11, 13-dihydro-8-deoxylactucin; 8 = 11, 13-dihydro-lactucopicrin; 9 = lactucopicrin. Peaks 1, 2, 5 and 10 = unknown.