Figure 7. IEC miRNA-deficiency is Associated with Exacerbated DSS Colitis and is Rescued Following WT Fecal RNA Transplantation.

(A to C) 6-wk-old gender-matched Dicer1^fl/fl and Dicer1^ΔIEC littermates were treated with 3% DSS in drinking water for 7 days. (A) Percentile change of body weight (BW). Linear regression curves of the BW change are shown at right panel (Values are mean ± SEM, p< 0.0001 between groups); (B) Colonic length (Values are mean ± SEM, p= 0.0003 between groups, t-test); (C) Histologic analysis (H&E) at day 9 post DSS administration (n = 8, represents two independent experiments). (D-G), (D) Schematic diagram of fecal RNA transfer and colitis induction: Donor fecal RNA was isolated from Dicer1^fl/fl (n=8) or Dicer1^ΔIEC (n=8) mice feces and was administrated by gavage to Dicer1^ΔIEC recipient mice, once daily (q.d.) for 7 days. Colitis was then induced by applying 3% DSS in drinking water for another 7 days. In the recipients, (E) Body weight change (Values are mean ± SEM, p< 0.0001 between groups); (F) Colonic length (Values are mean ± SEM, p= 0.0322 between groups, t-test); and (G) histologic analysis (H&E) at day 9 post DSS administration were analyzed.

Related to Figure S6.