Fig. 1.

Effect of RCB on adipocyte differentiation in 3 T3-L1 cells. a Lipid accumulation in 3 T3-L1 adipocytes assessed by Oil red O staining. 3 T3-L1 pre-adipocytes were differentiated into mature adipocytes and then treated with RCB for 7 days. At day 7 post-induction, the cells were fixed, and neutral lipids were stained with Oil red O. DMI, fully differentiated adipocytes (0.5 mM 3 IBMX, 100 μM indomethason, 0.25 μM dexamethasone and 167 nM insulin); 50, fully differentiated adipocytes (DMI + 50 μg/ml RCB); 150, fully differentiated adipocytes (DMI + 150 μg/ml RCB). RCB represents Rubus crataegifolius Bunge extracts. The scale bar is 50 μm. b Effect of RCB on TG accumulation in differentiating 3 T3-L1 cells. Triglyceride content was measured using a triglyceride assay kit. The results shown are representative of at least three independent experiments. The values are presented as the means ± SD. Different letters on the each bar graph indicate that the differences were statically significant (I 0.05) according to Duncan’s multiple range test. c Effect of RCB on cytotoxicity during 3T3L1 pre-adipocyte differentiation into adipocytes. 3 T3-L1 cells were treated with RCB at various concentrations (0, 50, or 150 μg/ml) in a DMI mixture for 4 or 7 days. Cell viability after treatment with RCB was determined with an MTT assay