Fig. 2.

Effects of RCB on the mRNA and protein expression of genes related to adipogenesis. a RCB inhibited the mRNA expression of adipocyte-specific factors during 3 T3-L1 adipocyte differentiation. Differentiation of 3 T3-L1 pre-adipocytes into adipocytes was induced using DMI media in the absence or presence of RCB (50 μg/mL or 150 μg/mL) for 5 days. β-actin expression in each sample was used as an internal control to normalize expression. The results shown are representative of at least three independent experiments. The different letters on each bar graph indicate significant differences (p < 0.05), as determined by Duncan’s multiple range test. b RCB inhibited the protein expression of adipocyte-related factors in 3 T3-L1 adipocytes. Cell lysates from the 3 T3-L1 cells were prepared at day 4 or day 7 after the induction of differentiation. Western blotting analysis was described in detail in the Materials and Methods section