Fig. 2.

Secretome of GIFT4-CLL cells. Primary CLL cells were treated with GIFT4 (Dark), GM-CSF and IL-4 (Dark gray), IL-4 (Light gray) or PBS (White) for 5 days. The treated cells were harvested, washed, and re-culture for 24 h in fresh complete RPMI-1640 medium. The culture supernatants were collected and subjected to cytokine luminex assay with human 51plex cytokine polystyrene bead kit. a–c Cytokine and chemokine concentrations were calculated from three independent experiments using samples from subjects No. 2, 3 and 5