Fig. 6.

Secretome and cytotoxic factors of GIFT4-CLL cell primed T cells. a Autologous T cells primed by GIFT4-CLL cells (GIFT4/T cells) (Dark), GM-CSF and IL-4 treated CLL cells (GM-CSF and IL-4/T cells) (Gray) or PBS-treated CLL cells (T cells) (White) were purified with T cell isolation kits and re-cultured in fresh RPMI medium. The T cell supernatants were subjected to luminex assay, and cytokine/chemokine concentration was calculated from three independent experiments. b Cytotoxic factors perforin, granzyme B and CD314 produced or expressed by GIFT4-CLL cell primed T cells (GIFT4/T cells) (Dark), or by GM-CSF and IL-4/T cells (Gray) or control T cells (White) were analyzed by FACS with intracellular staining of perforin, granzyme B or surface staining of CD314. Data were represented from one of three repeated experiments using samples from subjects No. 9, 10 and 11