Fig. 7.

Killing of primary CLL cells by GIFT4-CLL cell primed T cells. a, b Primary CLL cells were co-cultured with normal T cells (PBS T cells), or with T cells primed by GM-CSF and IL-4 treated CLL cells (GMCS + IL4 T cells), or with GIFT4-CLL cell-primed autologous cytotoxic T cells (GIFT4 T cells) (1:1 ratio) in absence or presence of perforin inhibitor concanamycin for 24 h. The cells were then harvested and stained with anti-human CD19 antibody and Annexin V, and subjected to apoptotic analysis by FACS. b Combined histogram of Annexin V positive primary CLL cells. c Alternatively, normal B cells isolated from healthy subjects were co-cultured with GIFT4-CLL cell-primed T cells for 24 h before subjected to FACS analysis with Annexin V. d Percentage of apoptotic death of primary CLL cells in the treated groups was calculated from three independent experiments using samples from subjects No. 4, 8 and 12