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. 2016 Apr 27;13:29. doi: 10.1186/s12977-016-0263-z

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© Shiohama et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Generation and characterization of anti-HTLV-1 bZIP factor (HBZ) monoclonal antibodies (mAbs). a HBZ protein levels in HBZ-transfected 293T cells were analyzed by indirect immunofluorescence using anti-HBZ mAbs P6-A7 (raised against peptide #1, mouse IgG2b), #20-H12 (raised against peptide #2, mouse IgG1), #91-1 (raised against peptide #3, rat IgG1), and #7-1 (raised against recombinant HBZ, mouse IgG2b). b HBZ expression vector-transfected 293T cells (HBZ) and mock-transfected 293T cells (Mock) were analyzed by western blotting using the novel anti-HBZ mAbs. c HBZ expression vector-transfected 293T cells (red line) and mock-transfected 293T cells (blue line) were analyzed by flow cytometry using the novel anti-HBZ mAbs. Three independent experiments were performed, and representative data are shown in each figure