Table IV.
ITC measurements of human galectin-7 with HMGs
| HMG | Ka (M−1 × 10−3) | Kd (mM) | ΔH (kcal/mol) | ΔS (cal/mol/K) | ΔG (kcal/mol) |
|---|---|---|---|---|---|
| Lactose | 2.88 (0.075)a | 0.348 | −10.695 (0.0050) | −20.1 | −4.72 |
| LNnT | 6.6 (0.40) | 0.15 | −11.8 (0.31) | −22.3 | −5.2 |
| LNT | 10.5 (0.32) | 0.0952 | −9.7 (0.15) | −14.1 | −5.48 |
| LNFPI | 9.8 (0.31) | 0.10 | −8.1 (0.13) | −8.8 | −5.4 |
| 2′-FL | 3.0 (0.18) | 0.34 | −8.7 (0.31) | −13.2 | −4.7 |
| 3-FL | n/ab | >1 | n/a | n/a | n/a |
aNumbers in parentheses represent the uncertainty. For lactose and LNnT, the uncertainty is the standard error of the mean (SEM) of two independent experiments. For all other HMGs, the uncertainties are the Origin 7-calculated curve-fitting uncertainties from a single experiment.
bn/a = data not available due to lack of measurable binding.