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. 2016 Apr 27;7:609. doi: 10.3389/fmicb.2016.00609

FIGURE 1.

FIGURE 1

Qualitative detections of total specific anti-GRAs IgG antibodies in mice sera. Western blots of purified recombinant proteins (rGRA2 or/and rGRA5) with sera of the immunized mice. Lane 1 contained PageRuler Prestained Protein Ladder. Lane 2–5 were incubated with sera of PBS-injected mice, lane 6–9 were incubated with sera of pRSET B-injected mice, lane 10–13 were incubated with sera of rGRA2-immunized mice and lane 14–17 were incubated with sera of rGRA5-immunized mice. The 4 sera represented sera collected at week 0, 2, 4, and 6 post-prime injections. The 30 kDa purified rGRA2 and 20 kDa purified rGRA5 were first detected at week 2 (lane 11 and 15 respectively) followed by an increase in the band intensity at week 4 and 6. No bands were observed in the mice sera of the two control groups.