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. 2016 Apr 27;7:609. doi: 10.3389/fmicb.2016.00609

FIGURE 3.

FIGURE 3

IgG isotypes determination in the immunized BALB/c mice sera. Polyclonal antibody isotypes (IgG1 and IgG2a) in the immunized mice sera were determined by ELISA. Sera from each mouse group were collected 2 weeks after the last injection. Data are expressed as mean OD450 ± SD (n = 3). Statistical difference is represented by ∗∗∗ (highly significant; p < 0.001) in comparison with the control groups (PBS or pRSET B).