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In vitro splenocytes proliferation response in mice. Spleen lymphocytes were harvested from mice immunized with rGRA2, rGRA5, pRSET B and PBS 2 weeks after last injection. The splenocytes were cultured and stimulated with the respective recombinant proteins. Proliferative response was measured by MTT assay. Data are expressed as mean stimulation index (SI) ± SD (n = 3). Statistical difference is represented by ∗(p < 0.05) in comparison with the control groups (PBS or pRSET B).
