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(A,B) IL-4 and IL-10 production by the stimulated splenocytes of the immunized mice. Culture supernatants from the antigen-stimulated immunized mice splenocytes were harvested at 24 and 72 h post-incubation for the evaluation of (A) IL-4 and (B) IL-10 production respectively via ELISA. Data are expressed as mean ± SD (n = 3). Statistical difference is represented by ∗(p < 0.05) in comparison with the control groups (PBS or pRSET B).
