Fig. 7.

Class II HDACs negatively regulate nSMase2 expression. MCF7 cells were seeded into 60 mm dishes and stimulated with vehicle (DMSO), TSA (300 nM), vorinostat (10 μM), LMK (5 μM), and mocestinostat (5 μM) for 24 h. A: Proteins were extracted in RIPA buffer and analyzed for Ac-H3 (K9), H3, and nSMase2 levels by immunoblot. Actin was used as loading control (*** P < 0.001, n = 4). B: RNA was extracted and converted to cDNA, and nSMase2 expression analyzed by qRT-PCR using actin as reference gene (*** P < 0.001, n = 5).