Fig. 2.
Strategies for testing the ability of chemical inhibitors to suppress the effects of RETMEN2 overexpression in Drosophila. a Eye-specific expression: ectopic overexpression of RETMEN2 under the control of the GMR promoter results in a fully penetrant rough eye phenotype in adults (red lines). Flies fed with vandetanib (ZD6474) strongly rescue defective eye development (blue line) [35]. b Expression in developing epithelia (wing, eye and leg) and other tissues: ectopic expression of UAS-RETMEN2 under the control of ptc-GAL4 results in lethality early in development (red line). This assay was used to screen a library of polypharmacological compounds capable of restoring viability (blue line). Further chemical refinement of initial hits identified a compound, AD80, with optimal efficacy and toxicity compared to analogs [37]. Colors refer to the online version only.