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. Author manuscript; available in PMC: 2016 Apr 27.
Published in final edited form as: Cell Rep. 2014 Oct 9;9(2):476–483. doi: 10.1016/j.celrep.2014.09.008

Fig. 4. The proline triplet of ValS is required for viability of E. coli.

Fig. 4

(A) Scheme for ValS complementation system. (B-G) Growth curves (OD600nm) of E. coli strains (B) BW25113, JL001, JL001 + pUlp1, or (C-G) JL001 bearing additional plasmids expressing pUlp1, and/or (C) wildtype ValS (pValS-PPP) or ValS mutants (D) pValS-GPP, (E) pValS-PGP, (F) pValS-PPG or (G) pValS-GGG. (H) Comparison of growth (after 90 min) of JL001 bearing plasmids expressing pUlp1 and pValS/XPP mutants normalized by subtraction of background growth in the presence of pUlp1 alone.