Table 1.
Antibodies in the phospho-CyTOF panel
| Phospho-CyTOF | ||||
|---|---|---|---|---|
| Metal label | Specificity | Clone | Source | Staining step |
| 139La | ||||
| 141Pr | ||||
| 142Nd | CD19 | HIB19 | DVS | Surface |
| 143Nd | ||||
| 144Nd | ||||
| 145Nd | CD4 | RPA-T4 | DVS | Surface |
| 146Nd | ||||
| 147Sm | CD20 | H1 | In house | Intracellular |
| 148Nd | ||||
| 149Sm | CD7 | CD7-6B7 | In house | Surface |
| 150Nd | ||||
| 151Eu | CD123 | 6H6 | DVS | Surface |
| 152Sm | CD27 | O323 | In house | Surface |
| 153Eu | CD45RA | HI100 | DVS | Surface |
| 154Sm | ||||
| 155Gd | ||||
| 156Gd | pp38 | D3F9 | DVS | Intracellular |
| 157Gd | CD24 | ML-5 | In house | Surface |
| 158Gd | pSTAT3 | 4/P-Stat3 | DVS | Intracellular |
| 159Tb | CD11c | Bu15 | DVS | Surface |
| 160Gd | CD14 | M5E2 | DVS | Surface |
| 161Dy | IgD | BD -1A6-2 | In house | Surface |
| 162Dy | pErk1/2 | Clone 20A | In house | Intracellular |
| 163Dy | IkBtot | CS L35A5 | In house | Intracellular |
| 164Dy | CD25 | M-A251 | In house | Surface |
| 165Ho | pS6 | Clone N7-548 | In house | Intracellular |
| 166Er | CD16 | B73.1 | In house | Surface |
| 167Er | CD38 | HIT2 | DVS | Surface |
| 168Er | CD8 | SK1 | DVS | Surface |
| 169Tm | pSTAT1 | BD -4A | In house | Intracellular |
| 170Er | CD3 | UCHT1 | DVS | Surface |
| 171Yb | ||||
| 172Yb | pSTAT5 | BD- 47 | In house | Intracellular |
| 173Yb | pPLCg2 | BD -K860689.37 | In house | Intracellular |
| 174Yb | HLA-DR | 1243 | DVS | Surface |
| 175Lu | CD56 | HCD56 | In house | Surface |
| 176Yb | CD127 | A019D5 | DVS | Surface |
| Qdot | ||||