Expression of fluorescent protein fusions of auxin transporters in transverse or longitudinal hand sections of basal inflorescence stem internodes from 5- or 6-wk-old plants. Patterns were consistent amongst all individuals examined, n > 10 for each line. Scale bars indicate ~30 μm. A)
PIN2:PIN2-GFP expression in the xylem parenchyma, longitudinal section. B)
PIN3:PIN3-GFP expression in the xylem parenchyma, longitudinal section. C–E)
PIN4:PIN4-GFP expression in pith, xylem parenchyma and cambium cells, transverse (C) and longitudinal sections (D,E) of 5-wk-old stems. F)
PIN7:PIN7-GFP expression in sup-epidermal cells, longitudinal section of 5-wk-old stem. G,J) Expression of PIN6:PIN6-GFP in basal internodes in transverse (G) and longitudinal (J) cross sections. Inset shows plasma membrane-localized PIN6-GFP in addition to probable ER-localized signal. H,K) Expression of PIN8:PIN8-GFP in basal internodes in transverse (H) and longitudinal (K) cross sections. I,L) Expression of ABCB19:GFP-ABCB19 in basal internodes in transverse (I) and longitudinal (L) cross sections. C = Cambium, P = Pith, SE = Sub-epidermis, X = Xylem, XP = Xylem parenchyma.