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. 2016 Apr 27;14(4):e1002446. doi: 10.1371/journal.pbio.1002446

Fig 8. PIN7 expression is dynamic during stem development.

Fig 8

A–D) PIN1:PIN1-GFP expression in the cambium and xylem parenchyma, transverse (A,C) and longitudinal sections (B,D) of basal (A,B) and apical (C,D) internodes of 30cm tall (= 6/7 wk old) inflorescence stems. E–F) PIN7:PIN7-GFP expression in transverse (E) and longitudinal sections (F) of basal internodes of 3 cm tall (= 5 wk old) inflorescence stems. G–J) PIN7:PIN7-GFP expression in transverse (G,I) and longitudinal sections (H,J) of basal (G,H) and apical (I,J) internodes of 15 cm tall (= 5/6 wk old) inflorescence stems. K–L) PIN7:PIN7-GFP expression in transverse (K) and longitudinal sections (L) of basal internodes of 30 cm tall (= 6/7 wk old) inflorescence stems. M) Summary of efflux carrier expression in Arabidopsis stems, showing PIN expression domains and localization patterns in a simplified longitudinal transect of cells in a vascular bundle and surrounding tissue. Tissue domains are indicated by red bars at the top. Expression domains of PIN1, PIN2, PIN3, PIN4, and PIN7 are indicated by colored bars at the bottom. ABCB19 is expressed in all tissues except the xylem and phloem (not shown). PIN proteins are not expressed (at least at detectable levels) in xylem or phloem vessels. PIN localization in cells is indicated by light green. PIN localization is apolar in pith and sub-epidermal cells and polar in other cell types, irrespective of the PIN protein type.