Figure 4.

Effects of panobinostat and MK-1775, alone or in combination, on cell cycle progression in CTS and U937 cells. Panel A: CTS and U937 cells were treated with 20 nM panobinostat, 250 nM MK-1775, or in combination for 24 h. Then the cells were fixed with ethanol, stained with PI, and subjected to flow cytometry analyses. Panel B: The percentage of cells in each cell cycle phase after drug treatment are presented as means of triplicates ± s.e.m from one representative experiment. *indicates p<0.05, **indicates p<0.005, and ***indicates p<0.0005.