Figure 2.

The effect of KLF2 overexpression on HCC growth in vitro (A) Representative protein levels of KLF2 in HuH7 cells transfected with KLF2 expression plasmids or empty vector (EV) for 24 hr. 25 μg protein lysis were used to perform western blot analysis. (B-D) Cell proliferation (B), cell-cycle progression (C) and invasion (D) assays in HuH7 cells expressing KLF2 or empty vector (EV). (E) Representative anchorage-independent growth activity for HuH7 cells with ectopic KLF2 expression and its corresponding control (EV). The colonies were observed at lower magnification (40X).