Figure 2.

Effect of 5-AcTMF on cell cycle progression and apoptosis in CL1-5 NSCLC cells. (A) Cells were treated with 2. 5 or 5 μM 5-AcTMF for 24 or 48 h, and cell cycle distributions were then analyzed by flow cytometry. The means ± SEM of the experimental triplicates is presented in the bar graph. (B) Apoptosis in CL1-5 cells was examined after 24 or 48 h of treatment with 2.5 or 5 μM 5-AcTMF by annexin V-FITC/PI binding and analyzed by flow cytometry. The means ± SEM of the experimental triplicates are presented in the bar graph. (C) Cells were treated with or without 5-AcTMF (5 μM) for 48 h and then lyzed for protein extraction. The cleave-caspase3, 8, 9 and PARP were examined by western blot analysis. GAPDH was used as a loading control. Densitometric analysis was performed using Image J software. The means ± SEM of three independent experiments are presented in the bar graph. *p < 0.05, **p < 0.01, ***p < 0.001, compared with the DMSO-treated group (one-way ANOVA).

Figure B.

(Continued).