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Pharmacologic inhibition of SPHK1 promotes the sphingolipid balance toward a pro-apoptotic state. NKL cells were treated in triplicate with 5 μM of SKI-178, 20 μM of SKI-II or DMSO vehicle control for 2 hours and lipids were extracted from 5 million cells. S1P and sphingosine (A) and ceramide (B) species, were quantified by tandem mass spectrometry, represented as pmol / μg of protein. *P < 0.05 indicates significant differences compared to DMSO treated cells (Student's t test).
