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. 2015 Nov 17;17(1):65–78. doi: 10.1080/15384047.2015.1108492

Figure 3.

Figure 3.

(A) MFE-296 cells were treated with the indicated concentrations of AP24534 (0.1 and 1 μM) or PD173074 (1 μM). FGFR2 was immunoprecipitated with FGFR2 antibody and FGFR2 kinase assay was performed as describe in Materials & Methods section. *, p < 0.01; **, p< 0.001; significantly different from vehicle control. (B, C) Cells were treated with indicated concentration of AP24534 or PD173074 for 48 h, and the phosphorylation of FGFR (Tyr653/654) and FRS2α (Tyr436) were assessed by Western blot analysis. Actin was measured for the confirmation of equal amounts of protein loaded.