Table 1. Functional categories enriched in RNA-seq comparison of E16.5 and E18.5 Tbx18 mutant compared to wild type UGS.
| Enriched Functional Categories | ||
|---|---|---|
| E16.5 | E18.5 | |
| Down-Regulated DEGs | Myofibril | Regulation of transcription |
| Muscle organ development | Zinc finger / zinc ion binding | |
| Muscle system process | Chromosome organization | |
| Muscle cell differentiation | mRNA processing | |
| Sarcoplasmic reticulum | Microtubule organizing center | |
| Cytoskeletal protein binding | Cell cycle | |
| Myosin complex | Ribonucleoprotein complex | |
| Cardiac muscle tissue development | Cellular response to stress | |
| Myogenesis | Programmed cell death | |
| Establishment of protein localization | ||
| Regulation of mesenchymal cell proliferation | ||
| Regulation of epithelial cell proliferation | ||
| Prostate gland growth | ||
| Skeletal system development | ||
| Serine threonine kinase activity | ||
| Regulation of apoptosis | ||
| Up-Regulated DEGs | Peptidase inhibitor activity | Ribosomal protein / ribosome |
| Extracellular region | Mitochondrion | |
| Defense response to bacterium | ncRNA metabolism | |
| Complement and coagulation cascades | Proteinaceous extracellular matrix | |
| Nucleosome assembly | Response to nutrient levels | |
| High density lipoprotein particle | Wnt superfamily | |
| NF-KB binding | ||
| Oxidative phosphorylation | ||
| Regulation of smooth muscle proliferation | ||
| Regulation of Ras GTPase activity | ||
The functions of genes that were significantly up-regulated or down-regulated in E16.5 and E18.5 Tbx18GFP/GFP compared to wild type urogenital sinus (S1 Table) were analyzed separately using the DAVID functional clustering algorithm [46]. The leading GO term in significantly enriched functional clusters are shown in order of their enrichment.