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| Gene | Primers and probes (5′→3′) | Source | Efficiency | R2 |
|---|---|---|---|---|
| CDH17 | sense: CACCCTTTTGGTCATCGGTAT | this study | 95.1% | 0.97 |
| antisense: CATCAGTTTCTCAGAGGCTTGACT | ||||
| OCLN | sense: CTCGTCTGGATAAAGAACTGGATGA | this study | 100.7% | 0.99 |
| antisense: CTCGTCTGGATAAAGAACTGGATGA | ||||
| ZO1 | sense: CTCAGTACAGCCAGGGTGCT | this study | 108.1% | 0.99 |
| antisense: TCCGGTTTGGACACTAATGAGTT |
