Table 1. PCR Conditions and locus summary statistics.
| Locus | Ta (˚C) | Ta (s) | MgCl2 (mM) | BSA (mM) | Allelic Richness | Ho | He | Missing |
|---|---|---|---|---|---|---|---|---|
| Cope9 (GACA)n | 56 | 15 | 1.5 | 0 | 13 | 0.403 | 0.787 | 0.217 |
| Cope12 (GT)nGG(GT)n | 56 | 15 | 2.5 | 0 | 9 | 0.342 | 0.657 | 0.150 |
| Cper26 (CA)8 | 46 | 45 | 2.5 | 2 | 14 | 0.504 | 0.715 | 0.222 |
| Cper92 (TG)9 | 49 | 15 | 2.5 | 0 | 24 | 0.278 | 0.718 | 0.197 |
| Cper99 (CA)7 | 46 | 45 | 2.5 | 2 | 10 | 0.191 | 0.406 | 0.063 |
| Cper103 (TG)7 | 46 | 45 | 2.5 | 2 | 7 | 0.590 | 0.619 | 0.073 |
| Cper119 (TG)15 | 53 | 15 | 2.8 | 0 | 33 | 0.570 | 0.920 | 0.120 |
| Cper163 (TG)12 | 65–57† | 15 | 2.5 | 2 | 19 | 0.137 | 0.612 | 0.258 |
| Cper184 (TC)19 | 65–57† | 15 | 1.25 | 2 | 50 | 0.632 | 0.834 | 0.161 |
| Cper188 (CA)9 | 53 | 15 | 2.5 | 0 | 24 | 0.392 | 0.726 | 0.124 |
†: indicates touchdown PCR reaction used; Ta (˚C): annealing temperature; Ta (s): annealing time. Missing is the proportion of individuals which failed to amplify at the given locus (complete information on individuals which failed to amplify at each locus and location combination in S1 Table).