Fig 3. PTK6 contributes to the cytokine cocktail TNFα/IFNγ induced barrier dysfunction and FoxO1 nuclear translocation in YAMC.

Post-confluent, mortalized WT or PTK6-/- YAMC monolayers were treated with vehicle control (0.1% BSA in PBS) or TNFα (100ng/ml) and IFNγ (500U/ml) 16 hours. A) Nuclei (blue), ZO-1 (green), and FoxO1 (red) were detected via confocal microscopy. B) Quantitation of the ratio of ZO-1 intensity in the cytoplasm over cell-cell junction from Fig 3A. C) Quantitation for nuclear FoxO1 intensity of FoxO1 stain in Fig 3A. D) Western blot indicating expression levels of ZO-1 in total cell lysates (please see S5 Fig for full blot scan). *p<0.05. Error bars represent SEM.