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Menin was partially washed off from the membrane fraction by hydroxylamine (HAM), but a serine palmitoylation inhibitor (IWP2) failed to influence the distribution of Menin in the membrane fraction. (A). A diagram of experimental procedures of biochemical fractionation of cells, including treatment with or without IWP2, or with or without HAM. (B). Immunoblot analysis of Menin in the cytosol, nuclear and membrane fractions.
