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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Nat Biotechnol. 2015 Oct 26;33(11):1173–1181. doi: 10.1038/nbt.3388

Figure 1. Generation of genetically matched hESCs and hiPSCs.

Figure 1

(A) Schematic for the generation of genetically matched hESC and hiPSC lines. (B) Overview of HUES2 and HUES3 derivatives used for RNA-sequencing. (C) Top panel shows bright images of hESC subclones, in vitro-differentiated fibroblasts, whereas the bottom panel shows hiPSC lines stained for alkaline phosphatase activity or OCT4 expression. Co-staining with DAPI confirmed nuclear expression of OCT4 (inset). (D) Heatmaps depicting DNA methylation (left) and gene expression (right) levels of key fibroblast-associated and pluripotency-associated genes in isogenic hESCs, in vitro-differentiated fibroblasts and derivative hiPSCs.