Figure 2.

Synaptic growth associated with enhanced minis is suppressed by synaptotagmin 4 (syt 4^BA1) and BMP receptor (wit) mutants. (A) Representative traces of spontaneous release recorded from syntaxin 1A mutant (w¹¹¹⁸;; syx^3-69) and control (w¹¹¹⁸) larval muscle 6 NMJs. (B) Representative images of muscle 6/7 NMJs of control (w¹¹¹⁸), syx^3-69 mutant (w¹¹¹⁸;; syx^3-69), syt 4^BA1 null (w¹¹¹⁸;; syt 4^BA1), and syx^3-69, syt 4^BA1 double mutant (w¹¹¹⁸;; syx^3-69, syt 4^BA1). (C) Mean synaptic bouton number measured for indicated genotypes. (D) Representative images of muscle 6/7 NMJs of the indicated genotypes. (E) Mean synaptic bouton number measured for control (w¹¹¹⁸;; Cpx^PE), cpx^SH1 null (w¹¹¹⁸;; cpx^SH1), control Syt 4^PE (w¹¹¹⁸;; Syt 4^PE), syt 4^BA1 null (w¹¹¹⁸;; syt 4^BA1), and syx^3-69, syt 4^BA1 double mutant (w¹¹¹⁸;; syt 4^BA1, cpx^SH1) (F) Representative images of muscle 6/7 NMJs from control (w¹¹¹⁸;; Cpx^PE), cpx^SH1 null, wit null (w¹¹¹⁸;; $\frac{{\mathit{wit}}^{A12}}{{\mathit{wit}}^{B11}}$), wit, cpx^SH1 double mutant (w¹¹¹⁸;; $\frac{{\mathit{cpx}}^{SH1},{\mathit{wit}}^{A12}}{{\mathit{cpx}}^{SH1},{\mathit{wit}}^{B11}}$), and wit heterozygotes in cpx null background (w¹¹¹⁸;; $\frac{{\mathit{cpx}}^{SH1},{\mathit{wit}}^{A12}}{{\mathit{cpx}}^{SH1}}$). (G) Mean synaptic bouton number measured for the indicated genotypes. Indicated comparisons were made using ANOVA with post hoc Tukey analysis. Mean ± SEM is indicated. Scale bars = 10 μm.