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. Author manuscript; available in PMC: 2016 Sep 27.

Published in final edited form as: J Mol Biol. 2016 Feb 6;428(6):1180–1196. doi: 10.1016/j.jmb.2016.01.031

Fig. 7 — A. H/D exchange for Lsm11 (amino acids 1-169) in the apo-state (red) and bound to an equimolar amount of FLASH 138N (blue) after 1 min incubation in D₂O buffer. Peptides analyzed in panel C are indicated with the arrows. B. Differential plot of H/D exchange presented in panel A. C. Time-dependent H/D exchange for indicated Lsm11 peptides. The ₃₀SFDPLL₃₅ peptide is shown in both the apo-state (red) and in a complex with FLASH 138N (blue). D. Localization of potential functional and structural elements within the N-terminal region of Lsm11 (amino acids 1-169), as predicted based on the H/D exchange and GST-pull down assays.

Fig. 7 — A. H/D exchange for Lsm11 (amino acids 1-169) in the apo-state (red) and bound to an equimolar amount of FLASH 138N (blue) after 1 min incubation in D₂O buffer. Peptides analyzed in panel C are indicated with the arrows. B. Differential plot of H/D exchange presented in panel A. C. Time-dependent H/D exchange for indicated Lsm11 peptides. The ₃₀SFDPLL₃₅ peptide is shown in both the apo-state (red) and in a complex with FLASH 138N (blue). D. Localization of potential functional and structural elements within the N-terminal region of Lsm11 (amino acids 1-169), as predicted based on the H/D exchange and GST-pull down assays.