Cryogenic TEM images of core particles before and after layering confirm the presence of a thin film. Cryogenic TEM of (a) uncoated, carboxy-modified latex particles; (b) purified, LbL-coated carboxy-modified latex (CML-[PLL/DXS]5); (c) uncoated, doxorubicin-loaded liposome; (d) purified, LbL-coated, doxorubicin-loaded liposome (Lipo-[PLL/DXS]4-PLL-HS).