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. Author manuscript; available in PMC: 2017 Feb 16.
Published in final edited form as: Adv Funct Mater. 2016 Jan 3;26(7):991–1003. doi: 10.1002/adfm.201504385

Figure 4.

Figure 4

The quantification of Cryo-TEM images confirm statistically significant increases in size consistent with LbL modification. (a) Comparison of the diameters of bare, carboxy-modified latex (CML) particles and LbL-modified CMLs indicate a statistically significant (P<0.0001) shift in the size distribution. Dashed line denotes the mean size. N = 103 for bare and 153 for LbL CML. (b) Similarly, comparison of the liposome membrane thickness reveals statistically significant (P<0.0001) increase in thickness after LbL modification. Dashed lines denote the mean thickness. N = 113 for bare and 298 for LbL liposomes. (c) Calculation of LbL film thickness from either Cryo-TEM data or dynamic light scattering data give statistically consistent results for both CML (13.3 ± 0.6 nm by Cryo-TEM and 12 ± 1 nm by DLS) and liposomal (2.65 ± 0.09 nm by Cryo-TEM and 2 ± 1 nm by DLS) substrates. Error bars represent SEM. These data highlight important changes in LbL film thickness due to substrate selection. Individual particle measurements were plotted as a histogram and the kernel density function was estimated using R Statistical Software. All statistical tests were performed using one-way ANOVA (alpha = 0.01), with the Bonferroni post-test, on PRISM graphing software.