Figure 5.

LbL nanoparticles prepared using the TFF-assisted method do not exhibit nonspecific cytotoxic effects in vitro. SKOV3 cells were incubated with poly(L-aspartic acid), herparin sulfate, and hyaluronic acid terminated LbL nanoparticles that were prepared by either the TFF method (solid, blue bars) or the conventional centrifugal method (striped bars). After 72 hours, the cellular viability was determined using the Cell-Titer Glo luminescence assay. The results were normalized relative to untreated controls, and subsequent analysis by one-way ANOVA failed to find any statistically significant changes in cellular viability for any formulation, regardless of preparation method.