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. 2015 Nov 30;17(2):151–162. doi: 10.1080/15384047.2015.1121345

Figure 2.

Figure 2.

HBx isoforms promote the expression and phosphatase activity of PP2Ac in HCC cells. (A) qPCR and (B) Western blot analysis of the mRNA and protein levels of PP2Ac in HCC cells transfected with vector or fl-HBx- or ct-HBx-expressing plasmid, respectively. (C) PP2A phosphatase activity assay was performed to determine the effect of HBx isoforms on PP2Ac activity. Bar graphs indicate means ± standard deviation from 3 independent experiments. *P < 0.05, **P < 0.01 vs. empty vector-tansfected cells. (D) Interactions between PP2Ac and HBx isoforms detected by co-immunoprecipitation assay. Upper panels: Cell lysates were immunoprecipitated with anti-HBx antibody and Western blotted with anti-PP2Ac and anti-HBx antibody. Lower panels: Cell lysates were immunoprecipitated with anti-PP2Ac antibody and Western blots detected with anti-PP2Ac and anti-HBx antibody. The representative Western blots are shown from 3 independent experiments.