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. 2015 Nov 30;17(2):151–162. doi: 10.1080/15384047.2015.1121345

Figure 3.

Figure 3.

Downregulation of PP2Ac suppresses the aggressiveness of HCC cells. (A) CCK-8 assay showed that downregulation of PP2Ac significantly reduced the proliferation of Huh7 cells within a 7-day period. (B) Colony formation assay was performed to assess the effect of PP2Ac downregulation on the colony formation of Huh7 cells after 2-3 weeks of culture. (C) PI staining analysis of cell cycle distribution in Huh7 cells transfected with indicated shRNAs. (D) Assessment of the effect of PP2Ac knockdown on the number of S-phase cells by BrdU incorporation assay. The relative BrdU incorporation level was determined and reported as percentage of control. (E) Apoptosis detected by annexin-V/PtdIns staining. (F) Wound-healing assay was done to investigate the effect of PP2Ac downregulation on the migration of Huh7 cells. Representative images are shown from 3 independent experiments. (G) Transwell invasion assay was done to examine the effect of PP2Ac downregulation on the invasiveness of Huh7 cells. Bar graphs indicate means ± standard deviation from 3 independent experiments. *P < 0.05 , **P < 0.01 vs. control shRNA-tansfected cells. shPP2Ac-A1 and shPP2Ac-A2 represent 2 different PP2Ac-targeting shRNAs.