Figure 6.

Overexpression of PP2Ac promotes the growth of HCC cells in vitro and in vivo. (A) CCK-8 assay showed that overexpression of PP2Ac significantly enhanced the proliferation of HepG2 cells within a 7-day period, compared to empty vector-transfected cells. (B) Colony formation assay revealed that PP2Ac overexpression significantly facilitated the formation of colonies after 2-3 weeks of culture. (C and D) Effects of PP2Ac overexpression on the growth of HepG2 xenograft tumors in nude mice (n = 3 mice for each group). Tumor volume was measured every 5 days and tumor volume-time curves were drawn (C). At the end of the experiment (30 days after cell injection, tumor xenografts were removed, weighted, and photographed (D). ^**P < 0.01 vs. empty vector group.